Manufacturers Recommended Semi-Annual Calibration Confirmation
Re-Issue date/Distribution: Tuesday, October 22nd, 2013/ALL SQA-V Users Subject: It is recommended that twice per year, the SQA-V Gold calibration be checked against the original factory calibration parameters. Although there are acceptable calibration ranges for the SQA-V, the system parameters may be close to the high or low end of the range and proactive maintenance…
Room Temperature on the SQA-V – Defined
BACKGROUND: Room temperature is a general term describing common indoor temperatures. It is usually in the range of 20 °C (68 °F or 293 K) to 25 °C (77 °F or 298 K)**http://en.wikipedia.org/wiki/Room_temperature OVERVEIW:The WHO 5th edition manual recommends assessing sperm motility at either room temperature or at 37 °C (with a heated microscope stage). “These…

Semen Sample Collection – WHO 5th Edition Recommendations
Medical Electronic Systems recommends that WHO 5th Edition (2.2 Sample collection) standards be followed for sample collection and processing for all automated and manual evaluation methods: 2.2.1 Preparation The sample should be collected in a private room near the laboratory, in order to limit the exposure of the semen to fluctuations in temperature and to…
The Influence of Red Blood Cells on the SQA-V Sperm Quality Analyzer
TECHNICAL BULLETIN UPDATE: Red Blood Cells in Semen Samples Analyzed by the SQA-V Instrument – (VERSIONS: All Human SQA-V GOLD, SQA-V and Spermalite Systems) Tuesday, June 18, 2013 BACKGROUND:The SQA-V utilizes frequencies of light optimized for the analysis of sperm cells only. Red blood cells (RBCs) are not ‘counted’ during the assessment of sperm cell…
Sample Mixing for Semen Analysis – WHO 5th Edition 2.4.1 (Pages 17 and 18)
For Manual and Automated Semen Analysis: The nature of the liquefied ejaculate makes taking a representative sample of semen for analysis problematical. If the sample is not well mixed, analysis of two separate aliquots may show marked differences in sperm motility, vitality, concentration and morphology. To be certain of obtaining reproducible data, the sample should…
QwikCheck™ Beads Compliance with WHO 3rd, 5th and 5th Edition Manuals
Issue date: January 31, 2013 BackgroundQwikCheck™ beads are an in-vitro use only external quality control material for automated and manual sperm counting systems. The beads are used as a tool to assess the accuracy and precision of the laboratory’s sperm counting methods by providing a known target value and +/- range. The beads were developed…

Treating Highly Viscous Samples in Compliance with WHO 5th
Issue date: Monday, November 26, 2012Attn: All SQA-V /QwikCheck™GOLD (WHO 4th & 5th compliant software) Background:The WHO 5th edition manual (page 13) describes the semen liquefaction as follows: “Immediately after ejaculation into the collection vessel, semen is typically a semi-solid coagulated mass. Within a few minutes at room temperature, the semen usually begins to liquefy…

SQA-V WHO 5th Edition Reference Ranges – Question and Answer
An excellent series of “Questions and Answers” that came through our Austrian Distributor regarding cutoffs and reference ranges: Question:We are presenting the SQA-V at a conference and I fear that the “reference” range cutoff values for morphology will be questioned. The technical bulletin you sent says there is high correlation between SQA and humans…..however, human…
SQA-V GOLD Morphology Algorithm Compliance (strict Kruger) Assessment Criteria
SQA-V GOLD Morphology Algorithm Compliance with WHO 4th and 5th (strict Kruger) Assessment Criteria Issue date: October 25, 2012Attn: All SQA-V/SPERMALITE/QwikCheck™GOLD (All WHO 4th & 5th compliant software) Background: The SQA-V GOLD system reports “Normal Morphology” in accordance with the WHO 4th or 5th edition manual criteria (based upon the selected SQA-V software version –…