2016 CAP Proficiency Instructions for SQA Systems

April 27, 2016
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TECHNICAL BULLETIN:  TESTING CAP PROFICIENCY SAMPLES ON SQA SEMEN ANALYZERS
For All SQA-V and SQA-VISION Systems Version 2.45 and above UPDATE: April 27th, 2016

BACKGROUND:
The College of American Pathologists (www.cap.org) provides a nationwide proficiency challenge for automated methods of semen analysis.  These results are peer reviewed against other SQA users providing an objective and efficient way to maintain proficiency.  MES recommends this survey as an unbiased appraisal of user proficiency and system performance. 

TESTING INSTRUCTIONS FOR SQA-V/SQA-V GOLD/SPERMALITE SYSTEMS:
NOTE: The CAP proficiency material should first be run as Stabilized Sperm in the “Control” mode and re-run in the Test New Patient “Fresh” Mode if a result of 0.0 is reported in Stabilized Sperm Control mode.

SETTING UP THE DEFAULTS:

  • From the main menu of the SQA-V/SQA-V Gold/Spermalite select: SERVICE > SERVICE DATA.
  • From the V-Sperm computer, navigate to SET UP > SQA-V > SQA-V DEFAULTS and click “CONTINUE”.  You will now see a set-up screen displayed on the PC.
  • On the bottom half of the setup screen, select the “STABILIZED SPERM” option and enter the following for Level 1 and Level 2:    
    • Lot Number = Your Sample Reference # | Target = 50 | Range = 50 | Date = Today’s date.
    • Click “APPLY” and wait a few moments for the information to transfer to the SQA-V.
    • NOTE: Do not forget to change your defaults back to Latex Beads after running your CAP sample!

TESTING YOUR PROFICIENCY SAMPLES ON THE SQA-V/SQA-V Gold/Spermalite:

  1. From the MAIN MENU of the SQA select: RUN CONTROLS > LEVEL 1 and allow system to calibrate.
  2. Mix the sample well by aspirating it in and out 10 times with a transfer pipette.  Avoid bubbles!
  3. Load the SQA-V testing capillary and check CLOSELY for bubbles then wipe the capillary tip clean.
  4. Insert capillary into the testing chamber when prompted to do so.
  5. If a sample result of 0.0 is received, re-run the sample as a FRESH sample.
  6. To Re-Run sample as FRESH, select “Test New Patient” from the main menu and enter the CAP sample number as the Patient ID.
  7. All other entries on the first screen may be skipped.
  8. On the second screen select “FRESH” under sample type and < 1 M/mL for WBC CONC. (all other entry fields may be skipped).
  9. Select YES when asked if sample is sufficient for complete testing > 0.5 M/mL.
  10. Allow system to calibrate and insert the capillary when prompted.
  11. Report the results received.
  12. Repeat Steps 1 – 11 for the second CAP proficiency material level.

TESTING INSTRUCTIONS FOR SQA-VISION SYSTEMS:

SETTING UP THE DEFAULTS:

  1. From the MAIN MENU of the VISION PC, select SETTINGS > PROIFICIENCY. 
  2. Enter a SAMPLE ID, DATE, and a NOTE (if necessary).
  3. Click SAVE.

TESTING YOUR PROFICIENCY SAMPLES ON THE SQA-VISION:

  1. Select QC / PROFICIENCY from the main MENU and touch TEST NOW on the desired option.
  2. Mix the sample well by aspirating it in and out 10 times with a transfer pipette.  Avoid bubbles!
  3. Load the SQA-V testing capillary and check CLOSELY for bubbles then wipe the capillary tip clean.
  4. Insert capillary into the testing chamber when prompted to do so.
  5. Report the results received.
  6. Repeat steps 1 through 5 for all samples.

DOWNLOAD THESE INSTRUCTIONS HERE